Amplified fragment length polymorphism (AFLP) and biochemical typing of Photobacterium damselae subsp. damselae
نویسندگان
چکیده
منابع مشابه
Photobacterium damselae subsp. damselae, a bacterium pathogenic for marine animals and humans
Photobacterium damselae subsp. damselae (formerly Vibrio damsela) is a pathogen of a variety of marine animals including fish, crustaceans, molluscs, and cetaceans. In humans, it can cause opportunistic infections that may evolve into necrotizing fasciitis with fatal outcome. Although the genetic basis of virulence in this bacterium is not completely elucidated, recent findings demonstrate that...
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Photobacterium damselae subsp. damselae (Pdd) is considered to be an emerging pathogen of marine fish and has also been implicated in cases of histamine food poisoning. In this study, eight strains isolated from mullets of the genera Mugil and Liza captured in the Ligurian Sea were characterized, and a method to detect histamine-producing Pdd from fish samples was developed. The histamine-produ...
متن کاملPhotobacterium damselae ssp. piscicida: detection by direct amplification of 16S rRNA gene sequences and genotypic variation as determined by amplified fragment length polymorphism (AFLP).
A PCR protocol for the rapid diagnosis of fish 'pasteurellosis' based on 16S rRNA gene sequences was developed. The procedure combines low annealing temperature that detects low titers of Photobacterium damselae but also related species, and high annealing temperature for the specific identification of P. damselae directly from infected fish. The PCR protocol was validated on 19 piscine isolate...
متن کاملFirst time isolation of Photobacterium damselae subsp. damselae from Caranx sexfasciatus in Persian Gulf, Iran
BACKGROUND AND OBJECTIVE Photobacterium damselae subsp. damselae is a marine pathogenic bacterium which causes disease in marine animals and human. This bacterium mostly found in coastal shallow seawater. So, the aim of this study was isolation and characterization of Photobacterium damselae subsp. damselae from edible fish of Persian Gulf, Bandar Abbas. MATERIAL AND METHODS Totally 100 fish ...
متن کاملOccurrence of Photobacterium damselae subsp. damselae in bivavlve molluscs from Northwest Spain
Photobacterium damselae subsp. damselae has been recognized as pathogen for poikilotherm and homeotherm organims. In this study we describe for the first time the isolation of this microorganism from Mytilus galloprovincialis in harvesting areas situated in the northwestern coast of Spain. The identity of the isolates as Ph. damselae subsp. damselae was confirmed by biochemical tests and multip...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2002
ISSN: 1364-5072,1365-2672
DOI: 10.1046/j.1365-2672.2002.01748.x